• Interaction

    AccNo. 44465 Score 0.49
    Name PM_10456673
    Kd 1.0

    Peptide

    AccNo. 44343
    Name YVAD
    Sequence YVAD
    44343_small
    Internalized no
    Is Motif no

    Interactor

    AccNo. 43965
    Name unknown

    Experiment

    AccNo. 44307
    Classification incorrect?
    Click the corresponding button to vote. Your vote
    is used to improve the automatic classification.
    Voting_motivation
    CA CVD DM APO ANG MI BD
    0.90 0.88 0.32 1.00 0.12 0.13 0.30 Vote
    plus plus plus plus plus plus plus Yes
    minus minus minus minus minus minus minus No
    Name PM_10456673
    Detection unspecified method, MI:0686
    Source Pubmed Text Id 10456673
    Journal Leuk Res. 1999 Aug;23(8):751-62.
    Title Prolactin blocks glucocorticoid induced cell death by inhibiting the disruption of the mitochondrial membrane.
    Authors Weimann E, Baixeras E, Zamzami N, Kelly P
    Text Prolactin (PRL) has been reported to inhibit dexamethasone (Dex) induced cell death. Nevertheless, the mechanism through which PRL exerts its protective effect is still not unravelled. Here, we analyse the effect of PRL at different stages of the glucocorticoid (GC) apoptotic pathway in PRL dependent cells (Nb2 cells). PRL blocks completely the GC induced loss of the mitochondrial transmembrane potential (delta psi(m)) and consequently phosphatidylserine (PS) exposure and loss of DNA content. Although PRL promotes an upregulation of the bcl-2 expression, simultaneous addition of PRL to GC fails to maintain even the normal levels of this anti-apoptotic protein. This finding excludes a critical role for bcl-2 in the PRL protective effect against GC. GC induced delta psi(m) disruption can be inhibited by the ICE-like inhibitor zVAD-fmk but not by ICE inhibitor tetrapeptide acetyl-Tyr-Val-Ala-Asp.chloromethylketone (YVAD-cmk) nor by caspase-3 inhibitor zDEVD. It can be speculated that PRL blocks delta psi(m) disruption by inhibiting an unknown caspase activated by GC.
    Mesh Terms Animals; Apoptosis/drug effects; Cysteine Proteinase Inhibitors/pharmacology; Dexamethasone/antagonists & inhibitors; Dexamethasone/pharmacology; Down-Regulation/drug effects; Endopeptidases/biosynthesis; Endopeptidases/drug effects; Enzyme Induction; Hydrolysis; Intracellular Membranes/ultrastructure; Membrane Potentials/drug effects; Mitochondria/ultrastructure; Nerve Tissue Proteins/biosynthesis; Nerve Tissue Proteins/drug effects; Poly(ADP-ribose) Polymerases/metabolism; Prolactin/pharmacology; Proto-Oncogene Proteins c-bcl-2/metabolism; Rats
PepBank has been developed and is maintained by http://ric.csb.mgh.harvard.edu