• Interaction

    AccNo. 21843 Score 0.86
    Name APATS_HSVSMC Environment in vitro / culture
    Kd 1.0 Organism Homo sapiens (human)

    Peptide

    AccNo. 21727
    Name APATS
    Organism N/A
    Constraint disulfide
    Sequence APATSIG
    21727_small
    Origin phage display
    Form phage
    Internalized no
    Unnatural no
    Imaging no
    Is Motif no

    Interactor

    AccNo. 21574
    Name HSVSMC
    Description human saphenous vein smooth muscle cells
    Organism Homo sapiens (human)
    Type cell

    Experiment

    AccNo. 21793
    Classification incorrect?
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    Voting_motivation
    CA CVD DM APO ANG MI BD
    0.50 0.83 0.87 0.92 0.60 0.74 0.67 Vote
    plus plus plus plus plus plus plus Yes
    minus minus minus minus minus minus minus No
    Name PD_11
    Detection filamentous phage display, MI:0048
    Source PDF Text Id 11
    Journal Mol Ther. 2004 Feb;9(2):198-208.
    Title Development of efficient viral vectors selective for vascular smooth muscle cells.
    Authors Work LM, Nicklin SA, Brain NJ, Dishart KL, Von Seggern DJ, Hallek M, B√ľning H, Baker AH
    Text The vascular smooth muscle cell (SMC) is integral to the pathogenesis of neointimal formation associated with late vein graft failure, in-stent restenosis, and transplant arteriopathy. Viral vectors transduce SMC with low efficiency and hence, there is a need for improvement. We aimed to enhance the efficiency and selectivity of gene delivery to human SMC. Targeting ligands were identified using phage display on primary human saphenous vein SMC with linear and cyclic libraries. Two linear peptides, EYHHYNK (EYH) and GETRAPL (GET), were incorporated into the HI loop of adenovirus (Ad) fibers and the capsid protein of adeno-associated virus-2 (AAV-2). Exposure of human venous SMC to EYH-modified (but not the GET-modified) Ad vector resulted in a significant increase in transgene expression levels at short, clinically relevant exposure times. Similarly, the EYH-modified AAV vector resulted in enhanced gene transfer to human venous SMC but not endothelial cells in a time- and dose-dependent manner. The EYH-modified AAV vector also enhanced (up to 70-fold) gene delivery to primary human arterial SMC. Hence, incorporation of EYH into Ad and AAV capsids resulted in a significant and selective enhancement in transduction of SMC and has implications for improving local gene delivery to the vasculature.
    Mesh Terms Adenoviridae/genetics; Adenoviridae/physiology; Capsid Proteins/genetics; Capsid Proteins/metabolism; Cells, Cultured; Cysteine Endopeptidases/metabolism; Dependovirus/genetics; Dependovirus/physiology; Genetic Vectors/genetics; Heparin/metabolism; Humans; Multienzyme Complexes/metabolism; Muscle, Smooth, Vascular/cytology; Muscle, Smooth, Vascular/virology; Organ Specificity; Peptide Library; Peptides/genetics; Peptides/metabolism; Proteasome Endopeptidase Complex; Protein Engineering; Protein Transport; Saphenous Vein
    References
PepBank has been developed and is maintained by http://ric.csb.mgh.harvard.edu